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Archive - Feb 2015

First Analysis of Large Cohort of Clinically Relevant DNA Duplications Throughout Entire Genome

Copy number variations (CNVs), which are deletions or duplications of large chunks of the genome, are a major cause of birth defects, intellectual disability, autism spectrum disorder, and other developmental disorders. Still, geneticists can definitively say how a CNV, once discovered in someone's DNA, leads to one of these conditions in only a very small fraction of cases. To aid in the interpretation of CNVs, researchers at Emory University School of Medicine have completed detailed maps of 184 DNA duplications found in the genomes of individuals referred for genetic testing. The findings have published online in the American Journal of Human Genetics (AJHG) and are currently available (see link below). "Ours is the first study to investigate a large cohort of clinically relevant duplications throughout the genome," says senior author Katie Rudd, Ph.D., Assistant Professor of Human Genetics at Emory University School of Medicine. "These new data could help geneticists explain CNV test results to referring doctors and parents, and also reveal mechanisms of how duplications form in the first place." Despite advances in "next-generation" DNA sequencing, the first step for patients who are referred to a clinical geneticist is currently microarray analysis. This is a scan using many probes across the genome, testing if someone's DNA has one, two, three, or more copies of the DNA corresponding to the probe (two is the baseline). From this scan, geneticists will have a ballpark estimate of where a deletion or duplication starts and ends, but won't know where the breakpoints are exactly. "In a few years, advances in sequencing will make it possible to routinely capture data on copy number variation and breakpoints at the same time," Dr. Rudd says. "But for now, we have to do this extra step."

DNA Nanoswitches Drastically Reduce Cost of Analyzing Biomolecular Interactions; Authors Offer to Supply Free Materials; Immediate Widespread Adoption of New Tool Is Possible

A complex interplay of molecular components governs almost all aspects of biological sciences - healthy organism development, disease progression, and drug efficacy are all dependent on the way life's molecules interact in the body. Understanding these bio-molecular interactions is critical for the discovery of new, more effective therapeutics and diagnostics to diagnose and treat cancer and other diseases, but previously, to achieve such understanding, scientists have been required to have access to expensive and elaborate laboratory equipment. Now, a new approach developed by researchers at the Wyss Institute for Biologically Inspired Engineering, Boston Children's Hospital and Harvard Medical School, promises a much faster and more affordable way to examine bio-molecular behavior, opening the door for scientists in virtually any laboratory world-wide to join the quest for creating better drugs. The findings were published in the February 2015 issue of Nature Methods. "Bio-molecular interaction analysis, a cornerstone of biomedical research, is traditionally accomplished using equipment that can cost hundreds of thousands of dollars," said Wyss Associate Faculty member Wesley P. Wong, Ph.D., senior author of the Nature Methods study. "Rather than develop a new instrument, we've created a nanoscale tool made from strands of DNA that can detect and report how molecules behave, enabling biological measurements to be made by almost anyone, using only common and inexpensive laboratory reagents." Dr. Wong, who is also Assistant Professor at Harvard Medical School in the Departments of Biological Chemistry & Molecular Pharmacology and Pediatrics and Investigator at the Program in Cellular and Molecular Medicine at Boston Children's Hospital, calls the new tools DNA "nanoswitches".

Reduced Activity of Housekeeping Gene (beclin1) Linked to Most Aggessive Form of Breast Cancer (Triple-Negative); Existing Approved Drugs Might Prove Effective

University of Texas (UT) Southwestern Medical Center scientists have identified a strong link between the most aggressive type of breast cancer and a gene that regulates the body's natural cellular recycling process, called autophagy. Based on analysis of two large breast cancer databases, reduced activity of an autophagy gene, beclin 1, was related to both a higher incidence of triple-negative breast cancer and a poorer prognosis for breast cancer patients. The study, published on January 16, 2015 in the online journal EBioMedicine, is the first to document a correlation between beclin 1 and triple-negative human breast cancer and validates research in mouse models. "We have potentially identified a new pathway to be targeted in the most aggressive, difficult-to-treat form of breast cancer," said Beth Levine, M.D., (photo), Director of the Center for Autophagy Research and a Howard Hughes Medical Institute (HHMI) Investigator at UT Southwestern. "These data suggest that decreased beclin 1 activity contributes to breast cancer and poor survival outcomes. As a result, therapies that increase beclin 1 activity in breast cancer may be beneficial." Triple-negative breast cancer, which accounts for 10 to 20 percent of breast cancer, is called such because the cancer's cells lack estrogen and progesterone receptors and also do not have an excess of the human growth factor receptor 2 (HER2) protein on their surfaces. Chemotherapy, the standard treatment, has been limited in its effectiveness against triple-negative breast cancer. "With low beclin 1 expression, you have up to a 35-fold higher risk of having triple-negative breast cancer. That's really strong," said Dr. Levine, who holds the Charles Cameron Sprague Distinguished Chair in Biomedical Science and is co-senior author of the study together with Dr.

Hibernation, Thermogenesis, Brown Fat, and BAT Activation: Elevated Transcript Level in Torpor Cycle Is Caused by Enhanced Transcript Stabilization; Temperature-Sensitive Mechanism Protects Subset of Transcripts from Bulk Degradation

During hibernation, animals cycle between torpor and arousal. These cycles involve dramatic, but poorly understood echanisms of dynamic physiological regulation at the level of gene expression. In each cycle, brown adipose tissue (BAT) drives periodic arousal from torpor by generating essential heat. We applied digital transcriptome analysis to precisely timed samples to identify molecular pathways that underlie the intense activity cycles of hibernator BAT. Paradoxically, a cohort of transcripts increased during torpor, paradoxical because transcription effectively ceases at these low temperatures. A new study, published online on January 27, 2015 in an open-access article in the journal eLife, shows that this increase occurs not by elevated transcription, but rather by enhanced stabilization associated with maintenance and/or extension of long poly(A) tails. The study further reported that mathematical modeling supports a temperature-sensitive mechanism to protect a subset of transcripts from ongoing bulk degradation instead of increased transcription. The authors reported that his subset was enriched in a C-rich motif and genes required for BAT activation, suggesting a model and mechanism to prioritize translation of key proteins for thermogenesis. The eLife article was entitled,”Enhanced Stability and Polyadenylation of Select mRNAs Support Rapid Thermogenesis in the Brown Fat of Hibernators.” The authors were scientists from the University of Colorado School of Medicine, the Colorado School of Mines, and the HudsonAlpha Institute for Biotechnology.

[eLife article]

Scientists ID Defective Cell Extrusion Mechanism by Which Pancreatic Cancer May Begin; Same Defect May Also Be Involved in Lung Cancer and Colon Cancer; Work Suggests Possible Effectiveness of FAK Inhibitors

Researchers at the Huntsman Cancer Institute (HCI) at the University of Utah, and at collaborating institutions, have found that defects in how cells are squeezed out of overcrowded tissue to die, a process called extrusion, may be a mechanism by which pancreatic cancer begins. From these findings, the scientists may have identified an effective way to reverse the defective extrusion's effects without destroying normal tissues nearby. The results were published online on January 26, 2015 in an open-access article in the journal eLife. The study focuses on the epithelia, tissues lining the cavities and surfaces of structures throughout the body, including organs such as the pancreas. It is already well-established that most solid tumors arise from this type of tissue. The HCI research team analyzed previous published microarray data and found that a receptor for the lipid sphingosine 1-phosphate (S1P2) that is critical for the extrusion process is significantly reduced in the most common type of pancreas cancer known as pancreatic ductal adenocarcinoma (PDAC), lung cancer, and some types of colon cancer--all aggressive cancers that resist treatment with chemotherapy. Focusing on cells from PDAC tumors, the team found that reduced S1P2 levels led to reduced extrusion and cell death rates. About 50% of the cells did not extrude and formed masses, while most of the remaining ones extruded underneath instead of outside the cell layer. "This kind of altered extrusion may be a common hallmark of invasive tumor types," said Jody Rosenblatt, Ph.D., co-author of the study, Associate Professor in the Department of Oncological Sciences at the University of Utah School of Medicine, and an HCI investigator.

Diet Quality and Nutrition Are Key Determinants of Mental Health; These Factors Should Now Be Embraced As Crucial by Psychiatric Community

Evidence is rapidly growing showing vital relationships between both diet quality and potential nutritional deficiencies and mental health, a new international collaboration led by the University of Melbourne and Deakin University, also in Melbourne, has revealed. In an article published online in The Lancet Psychiatry ton January 29, 2015, leading academics state that, as with a range of medical conditions, psychiatry and public health should now recognize and embrace diet and nutrition as key determinants of mental health. Lead author, Dr. Jerome Sarris (image) from the University of Melbourne and a member of the International Society for Nutritional Psychiatry Research (ISNPR), said psychiatry is at a critical stage, with the current medically-focused model having achieved only modest benefits in addressing the global burden of poor mental health. "While the determinants of mental health are complex, the emerging and compelling evidence for nutrition as a key factor in the high prevalence and incidence of mental disorders suggests that nutrition is as important to psychiatry as it is to cardiology, endocrinology, and gastroenterology," Dr. Sarris said. "In the last few years, significant links have been established between nutritional quality and mental health. Scientifically, rigorous studies have made important contributions to our understanding of the role of nutrition in mental health," he said. Findings of the review in The Lancet Psychology revealed that, in addition to dietary improvement, evidence now supports the contention that nutrient-based prescription has the potential to assist in the management of mental disorders at the individual and population level.

Sleep Improves and Structures Infant Memory; Sleep Spindle Brain Waves Found Crucial

There is no rest for a baby's brain - not even in sleep. While infants sleep they are reprocessing what they have learned. Working with researchers from the University of Tübingen, scientists from the Max Planck Institute for Human Cognitive and Brain Sciences in Leipzig, Germany have discovered that babies of the age from 9 to 16 months remember the names of objects better if they have had a short nap. And only after sleeping can the infants transfer learned names to similar new objects. The infant brain thus forms general categories during sleep, converting experience into knowledge. The researchers also showed that the formation of categories is closely related to a typical rhythmic activity of the sleeping brain called sleep spindles: Infants with high sleep spindle activity are particularly good at generalizing their experiences and developing new knowledge while sleeping. Sleep means much more than just relaxation for our brain. The flow of information from the sensory organs is largely cut off while we sleep, but many regions of the brain are especially active. Most brain researchers today believe that the sleeping brain retrieves recent experiences, thereby consolidating new knowledge and integrating it into the existing memory by strengthening, re-linking, or even dismantling neuronal connections. This means that sleep is indispensable for memory. The Max Planck researchers have found this to be the case even in infants and toddlers. The results of the new research were published online on January 29, 2015 in an open-access article in Nature Communications. The article was entitled, “Generalization of Word Meanings during Infant Sleep.”