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Archive - Dec 28, 2017

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Double Strike Against Tuberculosis: Beta-Lactone Inhibits Mycomembrane Biosynthesis & Enhances Effects of Antibiotics

In search of new strategies against life-threatening tuberculosis infections, a team from the Technical University of Munich (TUM), as well as Harvard University and Texas A&M University in the USA have found a new ally. They discovered a substance that interferes with the mycomembrane formation of the bacterium. It is effective even in low concentrations and when combined with known antibiotics their effectiveness is improved by up to 100-fold. Among the greatest challenges when treating life-threatening tuberculosis infections is the increasing resistance to antibiotics. But the pathogen itself also makes the life of doctors difficult: its dense mycomembrane hampers the effect of many medications. A team of scientists headed by Stephan A. Sieber, Professor of Organic Chemistry at TU Munich, has discovered a substance that perturbs the formation of this membrane significantly. The mycomembrane of the tuberculosis pathogen Mycobacterium tuberculosis consists of a lipid double layer that encapsulates the cell wall, forming an exterior barrier. Structural hallmarks are mycolic acids, branched beta-hydroxy fatty acids with two long hydrocarbon chains. The team hypothesized that similarly structured beta lactones could "mask" themselves as mycolic acid to enter the mycolic acid metabolic pathways and then block the decisive enzymes. In the context of an extensive search, the interdisciplinary team of scientists hit the bullseye with the beta lactone EZ120. It does indeed inhibit the biosynthesis of the mycomembrane and kills mycobacteria effectively. Using enzyme assays and mass spectroscopy investigations, Dr.

Biotech Journalist Will Climb Mount Everest to Support Cancer Research at Fred Hutchinson Cancer Center

Hi, I’m Luke Timmerman, a biotech journalist, and I am carrying my 80-pound training backpack up and down the hills of Seattle for a reason. I’m training to climb Mount Everest, the highest mountain in the world, in 2018. Why do this? Of course, I love mountains. But mostly, I’m doing it to support the top-notch research at the Fred Hutchinson Cancer Research Center. I’m doing it to support my hometown of Seattle, and I’m doing it to support science itself. As a biotech journalist for 15 years, I’ve had the privilege to meet scientists around the world doing amazing work. I see a cancer revolution happening. Immunotherapies are emerging that harness the power of the immune system to attack cancer cells much like the viruses and bacteria we fight off every day. Fast DNA sequencers and other sensitive instruments are making it possible to detect cancer earlier than ever before, when it’s most easily treated. Fred Hutch is at the leading edge of cancer cures. Their pioneering research is helping people with many types of cancer live longer, and lead better lives. We’re seeing just the beginning of what is possible. We can’t let up—especially during this time of so much thrilling progress. So I ask you to please give generously to this important cause at a crucial moment in time. Let’s take this all the way. DONATE TO “THE CLIMB TO FIGHT CANCER AT FRED HUTCH,” (http://engage.fredhutch.org/site/TR?px=1161441&fr_id=1551&pg=personal) and you’ll help scientist push to the top of the mountain—the cure. Donations are 100 percent tax-deductible, and Fred Hutch sends donors a receipt automatically. (This text was drawn from Luke’s publication, the Timmerman Report, with permission. Luke is the author of the award-winning biography of legendary scientist Leroy Hood, titled simply “Hood.”)

University of Wisconsin Study of Aortic Valves in Pigs Provides Key Insight into Calcific Aortic Valve Disease (CAVD) in Humans

The diminutive size of our aortic valve -- just shy of a -- belies its essential role in pushing oxygen-rich blood from the heart into the aorta, our body's largest vessel, and from there to all other organs. Yet for decades, researchers have focused less on damaged valves than on atherosclerosis, the gradual hardening of the blood vessels themselves. Thanks, in part, to pigs at the University of Wisconsin (UW)-Madison's Arlington Agricultural Research Station, scientists are now catching up on understanding the roots of calcific aortic valve disease (CAVD). "For a long time, people thought CAVD was just the valvular equivalent of atherosclerosis," says Kristyn Masters (see photo at end), PhD, a Professor of Biomedical Engineering at UW-Madison and Vice Chair of the department. "Today, we know that valve cells are quite unique and distinct from the smooth muscle cells in our blood vessels, which explains why some treatments for atherosclerosis, such as statins, don't work for CAVD, and why the search for drugs has to start from scratch." A team led by Dr. Masters has cleared a longstanding hurdle in that search with a study published online on December 27, 2017 in PNAS. The researchers teased apart, for the first time, the early cascade of events that may eventually cause stenosis, a severe narrowing of the aortic valve that reduces blood flow to body tissues and weakens the heart. The only current treatment for stenosis is valve replacement, which typically requires risky and expensive open-heart surgery. "Our study sheds new light on the differences between atherosclerosis and CAVD, especially in terms of bottleneck events that we can target with drugs," says Dr. Masters, whose work is supported by the National Institutes of Health and the American Heart Association.

Scientists Identify Signaling Hub That May Be Key to Cancer Metastasis

A University of Hawai'i Cancer Center researcher has identified how some cancer cells are made to move during metastasis. The research provides a better understanding of how cancer spreads and may create new opportunities for cancer drug development. Metastasis causes the deaths of 90 percent of cancer patients. The spread of cancer by metastasis is driven by a set of mutant proteins called oncogenes, which cause cancer cells to multiply uncontrollably and promote their ability to move. How oncogene activity specifically directs the increased movement and metastasis is highly complex and remains largely unknown. Joe W. Ramos, PhD, Deputy Director of the UH Cancer Center and collaborators focused on investigating how these oncogenes and related signals lead to dysregulation of normal processes within the cell and activate highly mobile and invasive cancer cell behavior. The findings, published online on December 26, 2017 in PNAS, define a mechanism in which the oncogenes turn on a protein called RSK2 that is required for cancer cells to move. The open-access article is titled “RSK2 Drives Cell Motility by Serine Phosphorylation of LARG and Activation of Rho GTPases.” Dr. Ramos and colleagues found that the RSK2 protein forms a signaling hub that includes proteins called LARG and RhoA. They show that turning on this signaling hub activates the movement of the cancer cells. These results significantly advance understanding of how cancer cells are made to move during metastasis and may provide more precise targets for drugs to stop cancer metastasis in patients where there are oncogenic mutations. "These new data are very exciting. Blocking cancer invasion and metastasis remains a central challenge in treating patients.

Evox Therapeutics to Collaborate with Boehringer Ingelheim on Exosome-Mediated Drug Delivery

Evox Therapeutics Ltd (“Evox” or the 'Company'), a leading exosome therapeutics company, announced on December 19, 2017 that it has entered into a research collaboration with Boehringer Ingelheim to investigate exosome-mediated delivery of RNAs with high medical relevance to targets for specific disease areas of focus to Boehringer Ingelheim. The collaboration is part of Boehringer Ingelheim's Research Beyond Borders (RBB) initiative that explores emerging science and technologies for and beyond its core therapeutic areas to create new opportunities in disease indications of high medical need. Exosomes are small, cell-derived vesicles. Evox combines its exosome engineering platform with highly specific targeting technology, to enable the development of natural delivery nanoparticles for the treatment of severe diseases. Under the terms of the agreement, Evox and Boehringer Ingelheim will perform comprehensive in vitro and in vivo research with Evox's exosome technology in return for undisclosed financial considerations. This research may help to pave the way for approaching therapeutic concepts in diseases with high medical need that are currently not amenable to therapeutic intervention. Upon completion of these studies, Boehringer Ingelheim will have the option to negotiate a license agreement to further develop RNA drug candidates using Evox's exosome-mediated delivery technology. Commenting on the announcement, Dr. Antonin de Fougerolles, Chief Executive Officer of Evox, said: "Evox is pleased to add Boehringer Ingelheim to its growing list of collaborators. The use of exosomes as drug delivery vehicles provides significant advantages over other delivery methods as they can carry therapeutic molecules to difficult-to-reach target tissues.