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Archive - May 20, 2017

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Second Day of ISEV 2017 Annual Meeting in Toronto, Canada

The second day of the International Society for Extracellular Vesicles (ISEV) annual meeting began with three simultaneous early-morning Meet the Experts sessions—one on “EV-Mediated Functional Delivery of Protein and Nucleic Acids,” one on “EV lipids and Lipidomics,” and the third on “Rigor and Reproducibility in EV Analysis.” These sessions are designed for experts to give brief opening talks and then to field questions from the audience. The session on “EV-Mediated Functional Delivery of Protein and Nucleic Acids” was chaired by Lucia Languino and featured the expert speakers Janusz Rak from McGill University and Raghu Kalluri from the MD Anderson Cancer Center. Dr. Rak spoke first and emphasized that, despite the unequivocal functional consequences on target cells, an understanding of the uptake mechanisms and post-cargo processing for EVs remains elusive. He gave examples illustrating that proteins (TF and EGFR) could be transferred via EVs to recipient cells and shown to have activity. He noted the work of Jan Lötvall demonstrating the transfer of RNA via exosomes and he also cited recent evidence that ds genomic DNA could be transferred by EVs. Dr. Kalluri then came forward and focused on the question of how efficiently EV phenomena observed in vitro occurs in vivo. He said that these phenomena are likely highly regulated and happen at a relatively low efficiency in vivo or we wouldn’t be what we are. He said there must be in vivo barriers that exist that we are now unaware of and that it is important to understand how these barriers are overcome in some cases. He noted that there are likely different exosomes from different cells and perhaps different exosomes from cells in different states. There is a “booming” heterogeneity that needs to be unraveled, he said.

ISEV 2017 Conference Opens in Toronto, Canada

Day 1 of the Sixth Annual International Society for Extracellular Vesicles (ISEV) conference May 18-21 in Toronto, Canada, was officially opened Thursday morning with brief remarks by ISEV President Andy Hill, PhD, and International Organizing Committee Chairperson Sumitsa Sahoo, PhD. Dr. Hill welcomed the largest group of ISEV attendees ever (just slightly under 1,000) from more than 50 countries and applauded the worldwide research effort on EVs that is gathering ever-greater momentum. Dr. Sahoo warmly welcomed everyone to Canada and noted that the country is celebrating its 150th anniversary this year. She foreshadowed the seven plenary address that would be given over the next three days of the meeting and noted that 30 different symposia and six Meet the Expert sessions would be held in addition to many poster sessions and satellite events. She also thanked the numerous meeting sponsors. She then declared the meeting officially open and called on Marca Wauben, Ph.D., to introduce the first plenary speaker, Philip Stahl, PhD, the Edward Mallinckrodt Jr. Professor Emeritus, Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri. But her introduction was extra-special as it began with her presenting Dr. Stahl with the prestigious ISEV 2017 Special Achievement Award for his work, in 1983, in first describing the “exosome secretion pathway” and demonstrating that multivesicular bodies (MVBs) release their vesicles into the extracellular milieu after fusion with the plasma membrane.Dr. Stahl’s research spans nearly five decades at Washington University in St. Louis. Initially focused on lysosomal enzymes and lysosome biogenesis, the Stahl Laboratory and its many students have made a number of seminal contributions to our understanding of endocytosis, membrane trafficking, and cell signaling.