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Archive - May 22, 2017

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ISEV 2017 Wraps Up in Toronto; Next Year Barcelona !

The final day of the highly successful International Society for Extracellular Vesicles (ISEV) 2017 Annual Meeting in Toronto, Canada, began with three simultaneous early-morning experts workshop sessions on “ExRNA Progress and Potential,” “EV Track,” and “Demonstration Workshop Analysis with FunRich.” The exRNA session was moderated by Louise Laurent, MD, PhD, from UC-San Diego, and included expert panel members, Aleksandar Milosavljevic, PhD, from Baylor College of Medicine, Kendall Jennsen, PhD, from TGen, and Jeffrey Franklin, PhD, from Vanderbilt University Medical Center. The sessions were designed to foster open interaction between the audience and the expert panelists, and Dr. Laurent framed the discussion by suggesting a focus on where we are now and what we need to make progress going forward. She noted that standardization is an ongoing effort with variability in methods, variability in reproducibility, and the existence of intrinsic, systematic biases in techniques that are reproducible. She indicated that standardization of collection, processing, and analytical methods is needed and standards that are developed need to be made available to the community. Dr. Milosavljevic remarked on the value of pipelines used by the exRNA Consortium and data sharing carried out through the exRNA Atlas (http://exrna-atlas.org/). Dr. Laurent mentioned the needs for single vesicle analysis and improved methods for vesicle enumeration and sizing. One audience member raised the question of methods to isolate exRNA from different body fluids and another contributed information on an effort undertaken in Australia to address some of the method standardization issues. He said that the Australian National Measurement Institute had asked 20 different labs to carry out size measurements and then analyzed the data to develop standard operating procedures.

Day 3 of ISEV 2017 Annual Meeting in Toronto, Canada

Day 3 of the International Society for Extracellular Vesicles (ISEV) 2017 annual meeting in Toronto, Canada, opened with three simultaneous early-morning Meet the Experts sessions—one on “In Vivo Imaging-Based Analysis of EV-Biological Activity,” one on “Vesicular and Non-Vesicular Pathways of Extracellular RNA Release,” and the third on "EV-Mediated Parasite-Host Interactions.” These sessions are designed for experts to give brief opening talks and then to field questions from the audience. The third session was chaired by Dr. Ana Claudia Torrecilhas of the Federal University of Sao Paolo, and the featured expert speakers were Dr. Rodrigo Soares, of the Instituto Oswaldi Cruz in Brazil, who focused on the Trypanosoma cruzi parasite, and Dr. Martin Olivier, of McGill University in Canada, who spoke on Leishmania. Dr. Soares noted how T. cruzi EVs are agonists of the TLR2 receptor and stimulate the production of NO, TNF-, IL-6, and MAP kinases, eliciting a generally proinflammatory response that is conducive to the survival and invasiveness of the intracellular parasite. Dr. Olivier described how the Leismania parasite insect vector, the sand fly, transmits Leishmania EVs with its bite and that these EVs exacerbate Leishmania pathology. In addition, some of the Leishmania EVs contain a virus called LRV1. This virus can infect other Leishmania and exacerbate their virulence. The virus is not infective without the EV coat. Leishmania has a pro-inflammatory effect initially to attract vulnerable cells and then to sustain the infection. At the end of the session, Dr. Torrecilhas emphasized the need to better characterize the composition of parasite EVs to help better understand their immunomodulatory effects.