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Urine miRNAs Found Mainly in Exosomes in Patients with Systemic Lupus Erythematosus (SLE); One Exosomal miRNA Distinguishes Acute Lupus Nephritis

Researchers from the INCLIVA Biomedical Research Institute in Valencia, Spain, together with collaborators, have shown that microRNAs (miRNAs) in the urine of patients with systemic lupus erythematosus (SLE) are found mainly in exosomes, and the most significant SLE-associate increase in a specific miRNA associated with SLE was found in miR-146a (100-fold increase) and this increase was specifically in patients with active lupus nephritis. Among the exosomal miRNAs tested, only the miR-146a discriminated the presence of active lupus nephritis. The research was published online on September 21, 2015 in PLOS ONE. The article was titled “Increased Urinary Exosomal MicroRNAs in Patients with Systemic Lupus Erythematosus.” In their work, the research team quantified specific miRNAs in the urine of patients with SLE (n = 38) and healthy controls (n = 12) by quantitative reverse-transcription PCR in cell-free urine, exosome-depleted supernatant, and exosome pellet obtained by ultracentrifugation. In the ontrol group, miR-335* and miR-302d were consistently higher in exosomes than in exosome-depleted supernatant, and miR-200c and miR-146a were higher in the cell-free fraction. In SLE patients, all urinary miRNAs tested were mainly in exosomes, with lower levels outside them (p<0.05 and p<0.01, respectively). The researchers noted that this pattern was especially relevant in patients with active lupus nephritis compared to the control group or to SLE patients in absence of lupus nephritis, with miR-146a being the most augmented (100-fold change, p<0.001). Among the exosomal miRNAs tested, only the miR-146a discriminated the presence of active lupus nephritis. The researchers concluded that urinary miRNAs are contained primarily in exosomes in SLE, and the main increment was found in the presence of active lupus nephritis. They said that their findings underscore the attractiveness of exosomal miRNAs in urine, a non-invasive method, as potential renal disease markers. The senior author of the PLOS ONE article is Dr. Joseph Redon and the corresponding author is Dr. Raquel Cortes.The image shows exosomes released from a cell.

[PLOS ONE article]