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Spider Silk Is Produced Artificially for First Time

Being able to produce artificial spider silk has long been a dream of many scientists, but all attempts have, until now, involved harsh chemicals and have resulted in fibers of limited use. Now, a team of researchers from the Swedish University of Agricultural Sciences and Karolinska Institutet has, step by step, developed a method that works. Today they report that they can produce kilometer-long threads that for the first time resemble real spider silk. The results were published in the journal Nature Chemical Biology. Spider silk is an attractive material–-it is well tolerated when implanted in tissues, it is light-weight but stronger than steel, and it is also biodegradable. However, spiders are difficult to keep in captivity and they spin small amounts of silk. Therefore, any large-scale production must involve the use of artificial silk proteins and spinning processes. A biomimetic spinning process (that mimics nature) is probably the best way to manufacture fibers that resemble real spider silk. Until now, this has not been possible because of difficulties to obtain water soluble spider silk proteins from bacteria and other production systems, and therefore strong solvents has been used in previously described spinning processes. Spider silk is made of proteins that are stored as an aqueous solution in the silk glands, before being spun into a fiber. Researcher Dr. Anna Rising (shown at left with Dr. Johansson) and her colleagues Dr. Jan Johansson and Dr. Marlene Andersson at the Swedish University of Agricultural Sciences and at Karolinska Institutet have previously shown that there is an impressive pH gradient in the spider silk gland, and that this well-regulated pH gradient affects specific parts of the spider silk proteins and ensures that the fiber forms rapidly in a defined place of the silk production apparatus. This knowledge has now been used to design an artificial spider silk protein that can be produced in large quantities in bacteria, which makes the production scalable and interesting from an industrial perspective. "To our surprise, this artificial protein is as water soluble as the natural spider silk proteins, which means that it is possible to keep the proteins soluble at extreme concentrations,” says Dr. Rising. To mimic the spider silk gland, the research team constructed a simple, but very efficient and biomimetic, spinning apparatus in which they can spin kilometer-long fibers only by lowering the pH.

"This is the first successful example of biomimetic spider silk spinning. We have designed a process that recapitulates many of the complex molecular mechanisms of native silk spinning. In the future, this may allow industrial production of artificial spider silk for biomaterial applications or for the manufacture of advanced textiles", says Dr. Rising.

Among the authors are also researchers from Donghua University (China), Universidad Politécnica de Madrid (Spain), University of Oxford (UK), KTH Royal Institute of Technology (Sweden), Uppsala University (Sweden), and Lund University (Sweden).

Two videos can be found on the university's website (

[Press release] [Nature Chemical Biology]