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Two Genes Responsible for Remarkable Difference in Flower Color of Nearby Snapdragons Identified; Islands of Divergence Established by Gene Flow & Multiple Selective Sweeps; Bee Pollination a Key Factor

Snapdragons are charming tall plants that flower in a range of bright colors. In Spain, where snapdragons grow wild, these flower colors show a remarkable pattern: When driving up a road from Barcelona to the Pyrenees, snapdragons of the species Antirrhinum majus bloom in magenta at the beginning of the road, before a population of yellow flowering snapdragons takes over - separated by just a two kilometer c long stretch in which flower colors mix. Such hybrid zones of snapdragons are quite infrequent; only a few others are known. But why don't the snapdragons mix, with yellow and magenta flowers growing together over a wide area? Dr. Nick Barton at the Institute of Science and Technology Austria (IST Austria), together with Dr. David Field, previously a postdoc in Dr. Barton's group and now Assistant Professor at the University of Vienna, collaborated with molecular geneticists at the John Innes Center in Norwich, UK, to investigate the causes of this pattern. In an article published online on October 8, 2018 in PNAS, the scientists report that they identified the genes responsible for flower color difference from DNA sequence data. The open-access article is titled “Selection and Gene Flow Shape Genomic Islands That Control Floral Guides.” "DNA sequencing is becoming cheaper and cheaper. But analyzing sequence data and interpreting the patterns seen is very hard,” Dr. Barton explains. “In this study, we used sequence data from Antirrhinum plants to locate the individual genes that are responsible for the difference in flower color across the hybrid zone." The researchers compared the genome sequences of 50 snapdragons of each color, and measured how much the sequences diverged between magenta and yellow snapdragon populations. By plotting a statistical measure of divergence between the two populations, they found "islands" in the genome that are more divergent between yellow and magenta snapdragons than the rest of the genome. In the snapdragons, these islands correspond to genes responsible for flower color. The new paper focuses on two of those genes, which determine the magenta pigment, and are located close together on the genome.

In their article, the authors state that they “show that selective sweeps likely raised relative divergence at two tightly-linked MYB-like transcription factors, leading to distinct flower patterns in the two subspecies.”

How the sharp difference between yellow and magenta populations is maintained was the subject of the PhD thesis by Tom Ellis in Dr. Barton's lab. Through observations, both in the field and in experiments at IST Austria, Ellis found that bees prefer to pollinate the most common color flowers in a population: in magenta populations, bees mostly pollinate magenta flowers, in yellow populations, bees mostly pollinate yellow flowers. This selection in favour of the most common type keeps the hybrid zone sharp, and prevents exchange of genes that are linked to the flower color genes.

In the current study, the researchers wanted to determine how the two snapdragon populations become different. They found two reasons why the snapdragon populations diverge at the flower color genes.

Firstly, selection has favoured new variants at the color genes that make the flowers more attractive to bees - causing these genes to sweep through the population, and leaving a sharp signal in the DNA sequences.

Secondly, the flower genes become barriers to gene exchange. Any genes located close to or even between the flower genes cannot easily be swapped between the populations, and so the region of genome around the genes that determine flower color become divergent.

"Even with abundant DNA sequence data, it is often difficult to find exactly why species are different. Our study is the culmination of years of work, combining fieldwork and population genetics with genetic crosses, and analysis of gene expression,” explains Dr. Barton.

(Image credit: David Field).

[Press release] [PNAS article]