Syndicate content

Upcoming Personalized Medicine 3.0 Conference—Targeting Cancer

“Personalized Medicine 3.0--Targeting Cancer” is a one-day conference and networking opportunity for health and industry professionals, educators, and scientists. The conference will focus on cancer--using genomic information to characterize tumors precisely and ensure the use of the most effective treatment regimens for individual patients with the fewest side effects. The organizers note that personalized medicine is poised to transform healthcare over the next several decades, and that it offers both the possibility of improved health outcomes and the potential to make healthcare more cost-effective. The conference will be held in San Francisco at San Francisco State University from 9 am to 7 pm on Tuesday, May 25, 2010. The two previous annual conferences on personalized medicine have been enormous successes and similar results are expected for this third conference. The organizers urge you to register early as space is limited and the registration fee is $249 until April 15, 2010. Registration includes a light breakfast, lunch, and a networking reception at the end of the day. Registration details and a preliminary program are available at the conference web site (http://personalizedmedicine.sfsu.edu/), as are additional details on the conference.

New Genetic Risk Factors Identified for Brain Aneurysms

In the largest genome-wide study of brain aneurysms ever conducted, an international team led by researchers at the Yale School of Medicine has identified three new genetic variants that increase a person's risk for developing this deadly disease. "These findings provide important new insights into the causes of intracranial aneurysms and are a critical step forward in the development of a diagnostic test that can identify people at high risk prior to the emergence of symptoms," said Dr. Murat Gunel, senior author of the report. "Given the often-devastating consequences of the bleeding in the brain, early detection can be the difference between life and death." The new study, the second by Yale researchers published within the last 15 months, brings to five the number of regions of the genome that have been found to contribute to the nearly 500,000 cases of this devastating disorder diagnosed annually worldwide. The researchers searched across the entire genome for changes in the genetic code that were shared more often by aneurysm patients than by unaffected individuals. The researchers determined that if persons carry all of the risk variants discovered by the Yale-led team, they are five to seven times more likely to suffer an aneurysm than those individuals who carry none. While these findings have transformed the understanding of the genetic risks for intracranial aneurysms, considerable work remains to be done, the researchers noted. "These five findings explain about 10 percent of genetic risk of suffering an aneurysm," Dr. Gunel said. "This is 10 percent more than we understood just a couple of years ago, but there is a long way to go."

Second Study Suggests Life-Extending Drug May Be Effective in Alzheimer’s

A second study, in a different mouse model, has shown that the pharmacological agent rapamycin, which has previously been shown to extend life span in mice, may prevent Alzheimer’s disease in humans. A bacterial product first isolated from the soil on Easter Island, rapamycin is already approved by the U.S. Food & Drug Administration to prevent organ rejection in transplant patients. The first of the two Alzheimer’s studies, was published online on February 23, 2010 in the Journal of Biological Chemistry (A. Caccamo et al.), and showed that rapamycin curbed the effects of Alzheimer’s in one mouse model. The new study, published on April 1 in PLoS ONE, showed similar effects in a completely different mouse model of Alzheimer’s. Both reports came from the University of Texas Health Science Center at San Antonio and collaborating institutions. The second report showed that administration of rapamycin improved learning and memory in a strain of mice engineered to develop Alzheimer's. The improvements in learning and memory were detected in a water maze activity test that is designed to measure learning and spatial memory. The improvements in learning and memory correlated with lower damage in brain tissue. "Rapamycin treatment lowered levels of amyloid-beta-42, a major toxic species of molecules in Alzheimer's disease," said senior author Dr. Veronica Galvan. "These molecules, which stick to each other, are suspected to play a key role in the early memory failure of Alzheimer's."

Songbird Genome Offers Clues to “Learned Vocalizations”

The first genome of a songbird (the male zebra finch), has been sequenced and has revealed secrets of the relatively rare ability to communicate through “learned vocalizations.” This ability has been documented in just a few other animals, including other songbirds, parrots, hummingbirds, bats, whales, and humans. The ability is lacking in chickens, the only other bird to have had its genome sequenced, and is also absent in female zebra finches. The current research indicates that the ability seems to depend, in part, on the extensive involvement of non-coding RNAs (ncRNAs). A major reason the researchers decided to study the zebra finch genome was the male bird's ability to learn complex songs from its father. At first, a fledgling male finch makes seemingly random sounds, much like the babble of human babies. With practice, the young bird eventually learns to imitate its father's song. Once the bird has mastered the family song, it will sing that song for the rest of its life and pass the song on to the next generation. Though female finches do perceive and remember songs, researchers suggest that their inability to learn songs may be due to differences in sex hormones, as well as chromosomal sex differences affecting the brain. The chicken and zebra finch genomes are similar in many ways. Both have approximately one billion DNA base pairs--roughly one-third the size of a human genome. However, researchers discovered that some genes associated with vocal behavior have undergone accelerated evolution in the finch. For example, they found a disproportionately high number of ion channel genes among the 49 genes in the finch genome that are suppressed, or turned off, in response to song. Human ion channel genes have been shown to play key roles in many aspects of behavior, neurological function, and disease.

Underexpressed Protein May Play Role in Down Syndrome

Contrary to conventional wisdom that the symptoms of Down syndrome are likely caused by an overabundance of certain proteins due to the additional copy of chromosome 21, scientists at Ohio State University and collaborators have found evidence that at least some of the symptoms may actually be associated with underexpression of a certain protein or proteins due to the presence of five microRNA genes on chromosome 21. MicroRNAs bind to messenger RNA and cause the inhibition of protein synthesis for that messenger RNA. Computer analysis revealed over 1,600 proteins that were potential targets of the five microRNAs on chromosome 21, all of which could cause problems in Down syndrome because they would be underexpressed. Based on other evidence, the researchers selected one of the protein genes (for methyl-CpG-binding protein 2, known as MeCP2) for further study. Among the reasons for selecting this gene was that it is known to be mutated in Rett syndrome, an inherited cognitive disorder. The researchers used just two of the five microRNAs on chromosome 21 for the experiments in this study, miR-155 and miR-802, to match the only microRNAs available in the genetically engineered mouse model of Down syndrome. First, the researchers made copies of the relevant microRNAs. In human brain cell lines, they manipulated levels of those two molecules to show the inverse relationship with MeCP2. If the microRNAs were overexpressed, the level of the MeCP2 protein went down. When the microRNAs were underexpressed, the protein levels went up.

Paired Drug Combination Kills Precancerous Colon Polyps

A two-drug combination destroys precancerous colon polyps with no effect on normal tissue, opening a new potential avenue for chemoprevention of colon cancer, according to a team of scientists at The University of Texas M.D. Anderson Cancer Center and INCELL Corporation. The drug regimen, tested so far in mouse models and on human colon cancer tissue in the laboratory, appears to address a problem with chemopreventive drugs--they must be taken continuously long term to be effective, exposing patients to possible side effects, said senior author Dr. Xiangwei Wu, associate professor in M.D. Anderson's Department of Head and Neck Surgery. "This combination can be given short term and periodically to provide a long-term effect, which would be a new approach to chemoprevention," Dr. Wu said. The team found that a combination of Vitamin A acetate (RAc) and TRAIL, (tumor necrosis factor-related apoptosis-inducing ligand), kills precancerous polyps and inhibits tumor growth in mice that have deficiencies in a tumor-suppressor gene. That gene, adenomatous polyposis coli (APC) and its downstream signaling molecules, are mutated or deficient in 80 percent of all human colon cancers, Dr. Wu said. Early experiments with APC-deficient mice showed that the two drugs combined or separately did not harm normal colon epithelial cells. Separately, they showed no effect on premalignant polyps. RAc and TRAIL together killed premalignant polyps, causing programmed cell death known as apoptosis. RAc, researchers found, sensitizes polyp cells to TRAIL. The scientists painstakingly tracked the molecular cascade caused by APC deficiencies, and found that insufficient APC sensitizes cells to TRAIL and RAc by suppressing a protein that blocks TRAIL. Before human clinical trials can be considered, Dr.

Protein Addition Helps Normalize Blood Glucose in Mouse Study of Type 2 Diabetes

When levels of free protein p85 were increased in the livers of severely obese, diabetic mice, researchers at Children’s Hospital Boston-Harvard Medical School and the University of Tokyo saw improved glucose tolerance and reduced blood glucose levels. The effect lies in the influence of p85 on the transcription factor XBP-1 (X-box binding protein 1), the scientists said. Under the influence of p85, XBP-1 normally moves to the nucleus and turns on genes for numerous chaperone proteins, which reduce stress on the endoplasmic reticulum (ER) by aiding and stabilizing the folding of proteins that are produced there and then dispatched to do their jobs in the cell. In previous work, the authors had shown that the brain, liver, and fat cells of obese mice have increased stress in the ER. In the presence of obesity, the ER is overwhelmed and its operations break down. This so-called "ER stress" activates a cascade of events that suppress the body's response to insulin, and is a key link between obesity and type 2 diabetes. Until now, however, researchers haven't known precisely why obesity causes ER stress to develop. Senior author Dr. Umut Ozcan and colleagues have now shown that XBP-1 is unable to function properly in obese mice. Instead of traveling to the cell nucleus and turning on chaperone genes, XBP-1 becomes stranded. Probing further, the researchers found the reason: XBP-1 fails to interact with p85, which is part of an important protein (phosphotidyl inositol 3 kinase or PI3K) that mediates insulin's effect of lowering blood glucose levels. Dr. Ozcan's group identified a new complex of p85 proteins in the cell, and showed that normally, when stimulated by insulin, p85 breaks off and binds to XBP-1, helping it get to the nucleus.

Amphibious Caterpillars Discovered in Hawaii

Scientists at the University of Hawaii have discovered the first-ever species of insect that are able to survive an entire life stage spent both above and below the water’s surface. In mountain streams across the islands of Hawaii, the researchers observed the larvae (caterpillars) of the moth genus Hyposmocoma feeding and breathing both underwater and away from streams on dry rocks. The scientists said that the caterpillars can breathe and feed indefinitely and equally well both above and below the water’s surface, and can mature either submerged or completely dry. The amphibious caterpillars possess no gills or plastron, common structures for underwater respiration in other insects. When submerged, the caterpillars likely rely on the direct diffusion of oxygen through the hydrophilic skin along their abdomens, the researchers said. Perhaps as a result of their need for direct diffusion, the caterpillars occur only in fast-flowing, well-oxygenated streams, the authors wrote, and quickly die in stagnant water. Genetic analysis of DNA from 89 species of Hyposmocoma indicated that the amphibious lifestyle is an example of parallel evolution; the analysis showed evidence of at least three independent invasions of the water by strictly terrestrial clades (evolutionary groups including a single ancestor and all its descendants), beginning more than six million years ago, before the current “high islands” existed (note: high islands are of volcanic origin and are distinguished from “low islands,” which are formed by sedimentation or uplifting of coral reefs). The authors noted that why and how Hyposmocoma, an overwhelmingly terrestrial group, repeatedly evolved unprecedented aquatic species is unclear, although there are many other evolutionary anomalies across the Hawaiian archipelago.

Nanotech Research May Ultimately Lead to Retinal Implants

Researchers at Tel Aviv University are making progress in work to merge retinal nerve cells with electrodes in the hope of someday being able to create retinal implants for people. But that goal is quite a ways off, said research leader Dr. Yael Hanein. Until then, her team’s current invention might be used by drug developers investigating new compounds or formulations to treat delicate nerve tissues in the brain. "We're working to interface man-made technology with neurons” Dr. Hanein said. "It can be helpful in in vitro and in in vivo applications, and provides an understanding of how neurons work so we can build better devices and drugs," she said. Her group has developed a spaghetti-like mass of nano-sized (one-millionth of a millimeter) carbon tubes, and using an electric current has managed to coax living neurons from the brains of rats to grow on this man-made structure (see image, courtesy of Tel Aviv University). The growth of living cells on the nano substrate is a very complicated process, Dr. Hanein said, but the cells adhere well to the structure, fusing with the synthetic electrical and physical interface. Using the new technology developed in Dr. Hanein's laboratory, graduate student Mark Shein has been observing how neurons communicate and work together. "We are attempting to answer very basic questions in science," Dr. Hanein explained. "Neurons migrate and assemble themselves, and using approaches we've developed, we are now able to 'listen' to the way the neurons fire and communicate with one another using electrical impulses. Listening to neurons 'talking' allows us to answer the most basic questions of how groups of nerves work together. If we can investigate functional neuronal networks in the lab, we can study what can't be seen or heard in the complete brain, where there are too many signals in one place."

Blocking miRNA Might Aid Healing of Chronic Wounds

New results indicate that targeting a specific microRNA (miR-210) with a drug that could be used topically on the skin might offer new strategies for treating chronic wounds, which are sometimes fatal and cost the U.S. health-care system an estimated $25 billion annually. Ohio State University researchers have discovered, in a new animal study, that the presence of miR-210 in wounds with limited blood flow lowers the production of a protein (E2F3) that is needed to encourage skin cells to grow and close over the wound. In a parallel experiment using human skin cells, the researchers silenced the miR-210 with an experimental drug and saw E2F3 protein levels rise. The skin cells multiplied as a result. The research involved wounds that are ischemic, that is, they heal very slowly or are in danger of never healing because they lack blood flow and oxygen at the wound site. These types of wounds affect approximately 6.5 million patients each year, and are common complications of diabetes, high blood pressure, obesity, and other conditions characterized by poor vascular health. "When blood supply is inadequate, many things are deficient at the wound site, including oxygen. That leads to a condition called hypoxia," said Dr. Chandan Sen, senior author of the study. "We have shown that hypoxia induces miR-210, which actually blocks the ability of the cells to proliferate, a step necessary for the wound-closure process.” This research was published online on March 22, 2010 in PNAS. [Press release] [PNAS abstract]

Syndicate content